Parhaat Bitesize Bio Podcastit (2024)

1
Multi-View Light Sheet Microscopy to Track Cell Lineages in Tissue Morphogenesis 56:51

11d ago56:51

56:51

A major question in developmental biology is understanding how cellular fate decisions are regulated precisely in space and time. Fortunately, we can now begin to observe these processes with modern imaging approaches, which allow the tracking of cell fate decision events and cellular rearrangements by live 3D time-lapse microscopy. This developmen…

1
Enhancing 3D Spatial Biology with AI: Simplified Insights for All 1:09:11

25d ago1:09:11

1:09:11

In this episode, learn how AI-powered segmentation, spatial analysis, and phenotyping can help you gain new insights for 3D images with complex morphological measurements featuring up to 15 biomarkers. See how to characterize tissue microenvironments and examine the differences between normal and disease tissues without the need to code or train De…

1
Accelerate Scientific Progress: The Power of Reproducibility, Collaboration and New Imaging Technologies 1:12:31

1M ago1:12:31

1:12:31

Imaging scientists have a critical educational role in improving rigor and reproducibility by sharing their technical expertise and providing intellectual contributions in all aspects of image-based science. They participate in creating, developing, and disseminating guidelines, resources, and tools to improve image-based research. In this episode,…

1
Inside Immunoassays: Best Practices for Accurate and Reliable Results 56:26

1M ago56:26

56:26

Immunoassays play an indispensable role in scientific research. They facilitate the detection and quantification of specific analytes, enabling in-depth investigations into disease mechanisms, biomarker discovery, and evaluation of the potential of therapeutic interventions. Sample selection and preparation are of utmost importance, as they have a …

1
Windows On Neurovascular Pathologies 1:03:18

2M ago1:03:18

1:03:18

The brain is an immune-privileged organ, meaning it is protected from the entry of pathogens, blood circulating factors, and immune cells by a physical barrier called the blood-brain barrier (BBB). But pathological events such as ischemia (stroke), trauma (traumatic brain injury), or tumor growth (gliomas and brain metastasis), activate the vascula…

1
Unleashing Fibrotic Secrets: Coupling the Power of Laser Microdissection with Mass Spec 45:41

2M ago45:41

45:41

Tissue heterogeneity is a significant challenge when analyzing proteins in tissue samples to identify disease states. In this episode, explore how combining laser microdissection with mass spectrometry (LCM-MS) on fixed and stained tissues lets you explore the protein networks within pathological features to shed light on their molecular functions.…

1
Virtual Reality Showcase for Stellaris: Leica’s Confocal Microscopy Platform 25:07

2M ago25:07

25:07

Join Advanced Workflow Specialists for a virtual reality showcase of Leica's confocal microscopy platform, STELLARIS. In this episode of Listen In, discover how to enhance your confocal imaging from performing multicolor experiments with clear, sharp images to capturing fluorescence intensity and lifetime-based information simultaneously. Watch the…

1
Tools & Techniques for the Study of Biologics & Antibody Drug Conjugates 47:57

2M ago47:57

47:57

The fundamental cellular mechanisms of internalization and trafficking are crucial to many areas of cell biology, especially the proper function of therapeutic antibodies. Antibodies intended for use as antibody-drug conjugates (ADCs) should specifically bind to target cells and rapidly internalize via endocytosis. However, the ability to study the…

1
Multiplex Annotated Tissue Imaging System (MANTIS®) 1:07:54

2M ago1:07:54

1:07:54

Join us on Listen In as we unveil a breakthrough method for imaging skin immune cells, crucial for accurate diagnoses and personalized treatments. Traditional imaging faces challenges like high tissue autofluorescence and spectral spillover. However, our latest episode introduces a game-changing solution using the Leica SP8 microscope coupled with …

1
Breaking Out of The Mold - A Panel Discussion of Alternative Careers 50:20

3M ago50:20

50:20

Join us on Listen In for an inspiring episode that explores alternative careers for scientists who want to combine their artistic skills with their passion for science. If you love science but feel that traditional lab life isn’t for you, this episode is a must-listen. In this episode, we're joined by a panel of individuals who have carved out uniq…

1
Five-Color STED With a Single Depletion Laser and Fluorescence Lifetime Phasor Separation 56:33

3M ago56:33

56:33

Dive into the innovative world of STED microscopy and explore how researchers are pushing the boundaries of super-resolution imaging in this episode of Listen In. Traditional STED microscopy achieves high resolution by using a doughnut-shaped depletion laser to selectively suppress fluorescence, but multicolor experiments often face challenges such…

1
Grant Writing: Everything but the Science 32:47

3M ago32:47

32:47

Dive into the often daunting world of grant applications. Securing funding is critical for any researcher, but the process can be filled with tricky tasks and complex requirements. Join us as we explore strategies to handle common challenges in grant applications with expert advice. Learn how to tackle issues like: • Career disruption • Reason for …

1
Live-cell Fluorescence Lifetime Multiplexing Using Organic Fluorophores 45:33

3M ago45:33

45:33

In this episode, we explore the cutting-edge techniques revolutionizing fluorescence microscopy. Traditional methods are limited to detecting three to four targets due to the spectral overlap of fluorophores. But now, scientists are breaking these barriers with fluorescence lifetime multiplexing. This innovative approach uses differences in fluores…

1
Stress in the Lab. Common Pitfalls and How to Avoid Them 49:19

4M ago49:19

49:19

Stress is a universal challenge, and scientists face unique pressures such as impostor syndrome, the demand for positive results, and career uncertainty. These stressors can be overwhelming and often feel beyond our control. In this episode of Listen In, we explore the various stresses that scientists encounter in their daily lab life and discuss e…

1
Visualizing Protein-Protein Interactions by Non-fitting and Easy FRET-FLIM Approaches 1:10:53

4M ago1:10:53

1:10:53

Understanding molecular interactions in living cells is one of the key elements to deciphering the molecular mechanisms underpinning most cellular functions. Förster resonance energy transfer (FRET) is the gold standard for studying protein-protein interactions. Fluorescence lifetime imaging microscopy (FLIM) allows for a straightforward quantifica…

1
Optimizing Nuclei Extraction & Counting for Single Cell Sequencing 46:44

4M ago46:44

46:44

Single cell sequencing samples must be prepared to the highest possible standard. Discover reliable techniques to prepare and validate nuclei samples for single cell sequencing. In this episode, learn how to perform essential quality control checks, including a quick and reliable method for quantifying nuclei, measuring extraction efficiency, and v…

1
How to Effectively Communicate Your Research 24:46

4M ago24:46

24:46

Join Kate Christian as she demystifies the process of effectively communicating your research in the latest episode of Listen In. She describes the impact of research communication in and out of the lab and provides some guides to explaining your research in various contexts, such as lab tours, community visits, and other public engagements. As wel…

1
Imaging GPCR Activity: Unravelling Mechanisms from Molecules to Morphology 1:08:22

5M ago1:08:22

1:08:22

Precise control of cellular communication and signaling is crucial for every physiological system, with G protein-coupled receptors (GPCRs, the largest family of signaling receptors) playing a central role as essential molecular sensors of a cell’s environment. Because of their diverse physiological and pathophysiological roles, GPCRs are also majo…

1
Critical Analysis Of Research Papers: Practical Examples 42:45

5M ago42:45

42:45

As a researcher, one of the most crucial skills to develop is the ability to perform an efficient yet thorough review of manuscripts. Manuscript reviewing requires specific skills and knowledge to properly assess published literature. By reading and reviewing papers, researchers can develop and hone these skills, which will help them identify and h…

1
New TauSTED Tools for Gentle Live Imaging at Nanoscale Resolution 1:04:16

5M ago1:04:16

1:04:16

This episode of Listen In showcases the latest tools and developments for multiplex STED imaging of living specimens at nanoscale resolution. Confocal imaging is a fundamental tool for studying the complex interplay of biomolecules, molecular machines, and higher-order cellular structures owing to its optical sectioning, sensitivity, and temporal a…

1
Finding The Ideal Hook for Your Science Story 37:25

5M ago37:25

37:25

Your research is important, but it can be hard to explain to others why it's important. In this episode of Listen In, you'll learn to find the ideal hook for your science story. This trick, borrowed from journalism, involves taking a step back and thinking about all the aspects of your research and where they might overlap with other topics that at…

1
Culture Media Preparation: Contamination Control and Retention of Critical Components 52:37

5M ago52:37

52:37

Contamination poses a significant risk in cell culture, jeopardizing research outcomes and incurring costly setbacks. This episode of Listen In gives you valuable insights into often overlooked areas of your filtration processes and will help you safeguard your sensitive cell cultures. Explore the critical importance of sterile filtration in divers…

1
Automate Your Pipetting — Unleash Your Potential 40:49

6M ago40:49

40:49

Despite the growing number of new technologies and automated instruments, routine liquid handling is still a manual process in many labs. The trend towards automation is gaining importance, not only with complex applications, such as NGS library preparation but also with simple yet repetitive liquid handling steps where automated pipetting makes se…

1
Cellular Crosstalk in Neurodevelopmental Disorders 1:03:20

6M ago1:03:20

1:03:20

Inspired by mutations associated with neurodevelopmental disorders, Prof. Dr. Silvia Cappello’s group at the Ludwig Maximilian University of Munich aims to understand the role of extracellular mechanisms essential for the correct development of the human brain. Their research focuses on intrinsic and extrinsic mechanisms contributing to the formati…

1
End-point PCR Set-up and Optimization: Best Practice 57:59

6M ago57:59

57:59

In this episode of Listen In, master your PCR set-up for unparalleled results. Check out this enlightening discussion of the crucial factors that influence the outcome of PCR experiments and discover the parameters you can optimize for better and more consistent PCR results. You will get reliable advice directly from PCR experts at Eppendorf on key…

1
Approaches for More Transparent, Rigorous, and Reproducible Research 48:52

6M ago48:52

48:52

Improving open science and research reproducibility is both a technical and a social challenge. But how do we do it? One way is to design, conduct, and report research that can maximize the impact of our individual and collective investment in research. Plus, changing the research culture to prioritize 'getting it right' over 'getting it published'…

1
Confirmation Bias and The Scientific Method II: Applying the Scientific Method 1:18:26

7M ago1:18:26

1:18:26

For many researchers, modern science has become about getting results rather than asking questions. Results mean publications, and publications mean more funding. However, results are not the objective of the scientific method, which involves proposing an answer to a question, designing experiments to determine whether the proposed answer is true o…

1
Confirmation Bias and The Scientific Method I: How to Avoid Confirmation Bias 56:54

7M ago56:54

56:54

For many researchers, modern science has become about getting results rather than asking questions. Results mean publications, and publications mean more funding. However, results are not the objective of the scientific method, which involves proposing an answer to a question, designing experiments to determine whether the proposed answer is true o…

1
Cryo-tomography to Volume EM: Explore with Hydra Bio Plasma-FIB 1:00:54

7M ago1:00:54

1:00:54

Volume electron microscopy or volume EM (vEM) was named one of Nature's Technologies to Watch in 2023. The new Thermo Scientific Hydra Bio PFIB integrates advanced cryo-TEM lamella preparation to volume EM workflows. In this episode of Listen In, see how the new Thermo Scientific Hydra Bio Plasma-Focused Ion Beam (PFIB) extends cryo technology to v…

1
The 12Rs Framework: Enabling High-quality Humane Research Involving Animals 51:59

7M ago51:59

51:59

In this episode of Listen In, see how emerging and global challenges of conducting ethical animal research have led to the 12Rs Framework to guide humane experimental techniques in animal studies and other research areas. The Principles of Humane Experimental Technique, the 3Rs (Replacement, Refinement, and Reduction), have been the mainstay of hum…

1
Elevate Your Research with Spatial Insights 57:14

8M ago57:14

57:14

In this episode of Listen In, learn more about how the convergence of AI technology and scientific discovery can revolutionize our understanding of biology and disease. Generating insights into tissue microenvironments is crucial to our understanding of normal and abnormal tissue development, such as during cancer progression. Spatial biology metho…

1
Unleash New Biological Insights with Innovative Glycoanalytics 53:21

8M ago53:21

53:21

Glycosylation is a prevalent modification of proteins known to play diverse roles across a wide range of biological processes. While glycoproteins are widely recognized as key molecular features involved in human health and disease, the analysis of protein glycosylation has historically been hampered by a lack of efficient and simple methods to cha…

1
Contamination and Recovery of PFAS in Analytical Methods Requiring Filter Membranes 49:51

8M ago49:51

49:51

Liquid chromatography-mass spectrometry (LC-MS) is a crucial technique in many life sciences, including proteomics, metabolomics, pharmaceutical analysis, clinical diagnosis, and forensic science. In this episode of Listen In, elevate your analysis and liquid chromatography. Learn what consumables, materials, and reagents can harm your LC-MS data f…

1
Label-free 3D Live Cell Imaging and Quantification using Holotomography 1:02:03

8M ago1:02:03

1:02:03

Holotomography has emerged as a helpful tool for imaging live specimens without additional pre-treatment, such as fixation, fluorescence labeling, and excitation. It can achieve long-term three-dimensional observations of live specimens for weeks without cellular damage caused by photoactivation. The high resolution (under 150 nm lateral) achieved …

1
Back to Buffer Basics: Everything You Need to Know About Buffers 57:09

9M ago57:09

57:09

Buffers are critical to nearly all our experiments. Poorly prepared buffers lead to failed Western blots, poor protein yields, flat binding curves—and wasted time. Understanding why you need buffers, knowing how to choose the right one, and troubleshooting buffer issues are fundamental skills you can apply to make all your experiments work better—a…

1
How to Write a Research Paper IV: Understanding the Publication Process 49:24

9M ago49:24

49:24

You have written a research paper. What now? Your research can only have an impact if someone reads it. So, you must submit your article to a journal for publication. In this episode of Listen In, learn the nuts and bolts of scientific publication with a step-by-step tour of the whole process. Explore how to select the right journal and what tools …

1
How to Write a Research Paper III: Common Problems and How to Overcome Them 1:01:34

9M ago1:01:34

1:01:34

The best way to solve a problem is to avoid it in the first place. While journals can and do reject articles for scientific reasons, they also reject articles for structural issues. By taking a few simple steps, you can learn how to circumvent these difficulties for a smooth publication process. In this episode of Listen In, we'll go through some o…

1
How to Write a Research Paper II: The Anatomy 1:01:54

9M ago1:01:54

1:01:54

All research papers have certain elements in common: Introduction, Methods, Results, and Conclusion. How can you structure your introduction to best frame the research question for your readers? Must the methods section read like a laundry list of reagents? Can you be dramatic in the results without losing objectivity? Is there any room in the conc…

1
How to Write a Research Paper I: Telling Your Story 1:08:27

9M ago1:08:27

1:08:27

Rarely do we consider writing a scientific paper as a creative exercise. But what if that could change? The best way to communicate anything is through stories. The scientific process has all the elements inherent in storytelling: a rich background, a problem or conflict to solve, and a resolution. In this episode of Listen In, we will reframe the …

1
Using Antisense to Characterize Nonsense: A Mouse Model of Progranulin-Deficient Dementia 42:09

10M ago42:09

42:09

Transgenic mouse models are important tools for testing therapeutic strategies. In this episode of Listen In, discover the benefits and essential considerations when characterizing and using transgenic mouse models to test therapeutic approaches to treat neurodegenerative diseases. Plus, explore a knock-in mouse model of frontotemporal dementia tha…

1
Visualizing Membrane Dynamics by Electron Microscopy 40:30

10M ago40:30

40:30

Explore how the latest electron microscopy techniques and volume imaging can capture fast, dynamic membrane and vesicle remodeling events during synaptic transmission at the ultrastructural level. Neurons communicate at specialized junctions, or synapses, via chemical messengers called neurotransmitters. Within a millisecond of neuronal stimulation…

1
Benefits of Imaging Data in Flow Cytometry Experiments: A Panel Discussion 41:43

10M ago41:43

41:43

Experience the transformative power of unbiased, data-driven cell analysis with access to over 25 image-derived label-free parameters, empowering you to easily assess sample quality, optimize gating strategies, and validate rare events. In this episode, learn how automated image analysis combined with flow cytometry can help improve data accuracy a…

1
Jump into 3D Organoids: Basic Culture Techniques and Advanced Applications 1:01:26

10M ago1:01:26

1:01:26

Organoids are three-dimensional in vitro cultures derived directly from patient tissues or induced pluripotent stem cells (iPSC) and embryonic stem cells. They self-organize to generate structures that resemble the tissue of origin and recapitulate the key physiological functions of the parental organ. In this episode of Listen In, explore the bene…

1
Ethical Research Practice III: Image Manipulation – What's Ok, and What's Not 59:36

11M ago59:36

59:36

As a scientific researcher, you must present your work in an unbiased, original, and representative way. Without due care and attention, it is easy to drift over the boundary of what is acceptable and slip into habits that are unacceptable within the scientific community. This episode of Listen In with Cristy Gelling explores image manipulation. Ma…

1
Ethical Research Practice II: Reporting Negative Results and Outliers 48:41

11M ago48:41

48:41

As a scientific researcher, you must present your work in an unbiased, original, and representative way. Without due care and attention, it is easy to drift over the boundary of what is acceptable and slip into habits that are unacceptable within the scientific community. This episode of Listen In with Karen O'Hanlon Cohrt explores publishing negat…

1
Ethical Research Practice I: Plagiarism – What It Is and How to Avoid It 59:54

11M ago59:54

59:54

As a scientific researcher, you must present your work in an unbiased, original, and representative way. Without due care and attention, it is easy to drift over the boundary of what is acceptable and slip into habits that are unacceptable within the scientific community. This episode of Listen In with Karen O'Hanlon Cohrt defines plagiarism and pr…

1
Everyday Culture Practice: Improving Reproducibility in Cell Culture 58:41

11M ago58:41

58:41

Eukaryotic cell cultures respond to the slightest influence. Apart from the risk of contamination, minimal changes in cultivation parameters can affect their viability, growth, and cell metabolism. In this episode of Listen In, discover the measures you can take to improve the reproducibility of your experiments by incorporating Good Cell Culture P…

1
Precision and Efficiency at Your Fingertips: Why Electronic Pipettes are the Future of Pipetting 51:52

1y ago51:52

51:52

Many scientists see the added value in using electronic pipettes and have already upgraded their pipette fleet. The benefits include: • Reduced strain on thumbs and arms by improving pipetting ergonomics. • Increased reproducibility of experiments with motor-operated pipetting cycles. • Easy and efficient handling of high sample throughput. In this…

1
Introduction to Liquid Handling for Next-generation Sequencing Workflows 1:04:03

1y ago1:04:03

1:04:03

Next-generation sequencing (NGS) has revolutionized the ‘omics’ era, with the rapid decoding of massive DNA sequences, supporting the investigation into various biological processes in human disease, genetic inheritance, immunity, cancer, and others. But generating high-quality NGS libraries is a labor-intensive process that requires experience, pr…

1
Essentials in Centrifugation—Better Safe than Sorry! 1:03:10

1y ago1:03:10

1:03:10

Centrifugation is the cornerstone of lab life, used in nearly every workflow, be it a routine task like pelleting cells or a dedicated assay for an important biomolecular target. That's why it's such a pain when they break down. But just because we use centrifuges every day doesn't mean there aren't important technical aspects to consider or no par…

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