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All About the qPCR Standard Curve: The Key to Good PCR Data
Manage episode 374910793 series 3391757
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#73 — PCR is a fundamental technique all biologists rely on, and, for qPCR, we can construct a standard curve that tells us how good or bad our primers are.
In this episode, learn all about qPCR standard curves, what they tell you about your primers, and what to try if something doesn't look correct on your standard curve.
Check out the corresponding online article for an example qPCR curve. [1] For more PCR essentials, download our free eBook that explains everything you need to know. [2] And to drill specifically into the efficiency of your PCR, read our article on determining qPCR efficiency. [3]
Resources:
1. The qPCR Standard Curve: The Key to Good qPCR Data. Available at: https://bitesizebio.com/31470/obligate-qpcr-standard-curve/
2. The Fundamentals of qPCR and RT-qPCR. Available at: https://bitesizebio.com/the-fundamentals-of-qpcr-and-rt-qpcr/
3. Important Considerations for Determining qPCR Efficiency. Available at: https://bitesizebio.com/3177/determining-qpcr-efficiency/
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